Prehliadanie podľa Autor "Stanko, Michal"

Teraz sa zobrazuje 1 - 2 z 2
  • Obrázok miniatúry
    Položka
    Deer keds (Diptera: Hippoboscidae) as potential vectors of bacterial and protozoan pathogens in Slovakia
    (Elsevier B. V. : Amsterdam, 2026) Švirlochová, Klaudia Mária; Víchová, Bronislava; Syrota, Yaroslav; Oboňa, Jozef; Heglasová, Ivana; Vargová, Lucia; Stanko, Michal; Csanády, Alexander; Čisovská Bazsalovicsová, Eva; Zubriková, Dana
    Deer keds are ectoparasitic flies of wild ruminants and are increasingly recognised as potential vectors of various pathogens. This study aimed to assess the prevalence of selected vector-borne pathogens in wingless deer keds collected from 13 red deer and 2 fallow deer, as well as in host-seeking keds across multiple localities in Slovakia. In total, 240 flies identified as Lipoptena spp. were obtained from cervids, and 542 host-seeking deer keds were collected. Individual flies were screened using molecular methods for the presence of Borrelia spp., Bartonella spp., Rickettsia spp., haemotropic Mycoplasma spp., Anaplasma phagocytophilum, piroplasmids, and Kinetoplastida. In wingless Lipoptena spp., high DNA prevalence rates were detected for Bartonella spp. (88.33%), piroplasmids (60.83%), Anaplasma phagocytophilum (24.58%), and Kinetoplastida (20.42%). Haemotropic Mycoplasma spp. DNA was confirmed in 4.56% of flies, while DNA of Borrelia spp. and Rickettsia spp. was not detected. Among host-seeking keds, Bartonella spp. DNA was detected in 7.38%, Rickettsia spp. DNA in 0.92%, and haemotropic Mycoplasma spp. DNA in 0.37%, whereas the remaining pathogens were not detected. Regression analysis indicated significantly greater pathogen-group richness in deer keds collected from cervids, as host-seeking keds harboured an average of 1.76 fewer pathogen types than host-derived individuals. The probability of detecting Bartonella spp. and haemotropic Mycoplasma spp. was markedly lower in host-seeking keds. Overall, this study highlights the epidemiological relevance of Lipoptena spp. and underscores the need for further research on their vector competence and potential implications for animal and public health.
  • Obrázok miniatúry
    Položka
    Diversity and prevalence of spotted-fever group rickettsiae in ixodid ticks across Slovakia, Central Europe
    (Elsevier GmbH : Mníchov, 2025) Heglasová, Ivana; Víchová, Bronislava; Stanko, Michal
    Habitats with the sympatric occurrence of several ixodid tick species are significant from an epidemiological perspective. These habitats can influence the diversity and prevalence of tick-borne pathogens, and their monitoring can help estimate the risk of infection. A total of 1260 questing ticks from five species (Ixodes ricinus, Dermacentor marginatus, Dermacentor reticulatus, Haemaphysalis concinna, and Haemaphysalis inermis) were collected from vegetation using the flagging method in three different habitats in eastern Slovakia. Additionally, 900 rodent-attached ticks of six species (I. ricinus, Ixodes trianguliceps, D. marginatus, D. reticulatus, H. concinna, and H. inermis) collected from 149 small mammals belonging to seven species (Apodemus flavicollis, Apodemus agrarius, Microtus arvalis, Myodes glareolus, Micromys minutus, Crocidura leucodon, and Crocidura suaveolens) were selected for molecular analyses. DNA obtained from rodent-attached and questing ticks was tested by nested PCR targeting the gltA gene to determine the presence of Rickettsia spp. The ompA, ompB, and sca4 genes were amplified and sequenced to identify rickettsiae species. The overall prevalence of rickettsiae in questing and rodent-attached ticks was 12.5 % and 20.0 %, respectively. Overall studied localities, the most diverse spectrum of rickettsiae species, including R. helvetica, R. monacensis, R. raoultii, and R. slovaca, was recorded in questing and rodent-attached ticks in the natural habitat of the Slovak Karst. The dominant species, R. helvetica (62.9 %), was identified in two species of questing and rodent-attached ticks, specifically I. ricinus and H. concinna, and in rodent-attached D. reticulatus ticks. Rickettsia raoultii (20.4 %) was identified in questing and rodent-attached D. marginatus, D. reticulatus, and in questing H. concinna. Additionally, this study provides the first input of pathogenic R. raoultii in I. trianguliceps obtained from the striped field mouse (A. agrarius). Rickettsia monacensis (9.0 %) was detected in questing and rodent-attached I. ricinus, while Rickettsia slovaca (3.6 %) was found in questing and rodent-attached D. marginatus ticks. Natural biotopes with different tick species and hosts significantly affect the diversity and prevalence of rickettsiae.