Prehliadanie podľa Autor "Vesteg, Matej"

Teraz sa zobrazuje 1 - 5 z 5
  • Obrázok miniatúry
    Položka
    Analytical evaluation of PAH degradation during the cultivation of hydrocarbon-utilizing bacteria with bioremediation potential
    (Univerzita Pavla Jozefa Šafárika v Košiciach, 2025) Kupcová, Elena; Ševčíková, Janka; Krempaská, Vladimíra; Vesteg, Matej; Murgašová, Michaela; Guziurová, Pavlína
    Polycyclic aromatic hydrocarbons (PAHs) are persistent environmental pollutants characterized by high toxicity and low biodegradability [1]. In this study, bacterial strains capable of utilizing PAHs [2,3] were isolated from various extreme environmental habitats. The isolates were cultivated in mineral media supplemented with selected PAHs (specifically fluorene and phenantrene) as the sole carbon source and exposed to a range of concentrations to determine the maximum levels that the bacteria were able to utilize. The degradation efficiency was assessed by monitoring the residual concentrations of the target compounds using high-performance liquid chromatography with diode array and fluorescence detection (HPLC-DAD/FLD). The results revealed that several bacterial strains exhibited a remarkable ability to degrade specific PAHs even at elevated concentrations, highlighting their potential application in bioremediation of contaminated environments.
  • Obrázok miniatúry
    Položka
    Bacterial inhabitants of wood-decay fungi basidiocarps
    (Univerzita sv. Cyrila a Metoda v Trnave, 2025) Beck, Terézia; Vesteg, Matej; Ševčíková, Janka; Kureková, Janka; Pristaš, Peter
    Various bacteria were isolated from 14 basidiocarps of wood-decay fungi collected from June to September 2023 in selected locations in Slovakia (Oravské Veselé village, Banská Bystrica city, and a mixed forest in Vrútky city). Fungi were identified by combination of morphological traits and ITS spacer region sequence analysis, bacteria were identified by MALDI-TOF MS. To detect the cellulolytic ability of bacterial isolates, the Congo-Red Staining method with carboxymethyl cellulose medium was used. Highly variable populations of bacteria were detected in all basidiocarps with predominance of Pseudomonas spp. Most basidiocarps (9) were identified as Phellinus igniarius, from which multiple Pseudomonas spp. including P. extremorientalis, P. synxantha, two Achromobacter spp., Bacillus pumilus, B. subtilis, Enterobacter asburiae, E. bugandensis, Ewingella americana, Lelliottia amnigena, Serratia liquefaciens, and Stenotrophomonas sp. were isolated. From Trametes versicolor basidiocarps, Ochrobactrum sp. and Lelliottia amnigena were isolated; from Trametes gibbosa, Bacillus sp., Bacillus subtilis, Lysinibacillus fusiformis, two Pseudomonas spp., and Stenotrophomonas sp.; from Trametes hirsuta, three different Pseudomonas species and Raoultella terrigena were isolated. In Fomitopsis betulina, two Bacillus spp. and Sporosarcina sp. were detected. Cellulolytic activity was observed in bacteria belonging to the Pseudomonas, Bacillus, and Achromobacter genera. The possible role of fungi-bacteria symbiosis in wood-decay is discussed.
  • Obrázok miniatúry
    Položka
    Bokashi composting as an alternative method of processing biodegradable kitchen waste in households
    (Univerzita sv. Cyrila a Metoda v Trnave, 2025) Ševčíková, Janka; Laceková, Karolína; Murgašová, Michaela; Beck, Terézia; Vesteg, Matej
    The study focuses on the application of Bokashi composting as an alternative method of biodegradable kitchen waste (BKW) management in households. A questionnaire survey conducted in the Lučenec district aimed to assess residents’ willingness to separate BKW and their interest in alternative processing methods. The Bokashi composter uses anaerobic fermentation to transform waste into solid residues and fermentation liquid. The effectiveness of this liquid was tested via a phytotoxicity test using garden cress seeds (Lepidium sativum L.), observing germination effects at various dilutions over 48 hours. Results showed that 38.9% of respondents would prefer home composting with provided composters, and 61.1% consider it the most efficient method of BKW management. While 50% had not heard of the Bokashi method, 22.2% expressed interest in further information. Findings suggest a significant potential for domestic composting, especially when proper technical support and education are provided. The phytotoxicity test confirmed that the fermentation liquid is suitable for gardening use when adequately diluted.
  • Obrázok miniatúry
    Položka
    Determination of microbial isolates from two different composts and the evaluations of their potency to stimulate the growth of tomato
    (Univerzita sv. Cyrila a Metoda v Trnave, 2025) Murgašová, Michaela; Krempaská, Vladimíra; Beck, Terézia; Ševčíková, Janka; Vesteg, Matej
    Composting is an aerobic process consisting of several phases, producing a stable and mature fertiliser. The first compost analysed in this study originates from the municipal composting plant, which serves for decomposition of kitchen waste, while the second compost is being commercially sold. The aim of this study was to isolate bacteria promoting plant growth from both composts. Samples from composts were incubated at 25 °C in various liquid cultivation media. Subsequently, selected colonies were inoculated onto fresh solid media and the bacterial composition of both composts was analyzed by MALDI TOF-MS after protein extraction. We were able to isolate several interesting bacterial species including Lysinibacillus xylanilyticus and Solibacillus silvestris from municipal compost. Pseudomonas citronellolis and Pseudomonas stutzeri were isolated from the commercially sold compost. These isolates have traits that are specific for plant-growth promoting bacteria or they can serve as biocontrol agents against infections causing many plant diseases. Isolates with these traits can thus significantly facilitate the growth, development or immunodefence of tomato (Solanum lycopersicum L.).
  • Obrázok miniatúry
    Položka
    Diversity of psychrophilic bacteria isolated from cold alpine lakes in High Tatras, Slovakia
    (University of Belgrade, 2025) Krempaská, Vladimíra; Vesteg, Matej
    Psychrophilic bacteria are adapted to thrive at low temperatures through specialized cellular mechanisms, including the production of cold-active enzymes, antifreeze proteins and modified membrane lipids. These traits make them promising candidates for biotechnological applications in cold-chain industrial processes, biodegradation of pollutants in cold ecosystems and bioremediation of contaminated areas under low-temperature conditions. This study explores the bacterial diversity in selected glacial lakes of High Tatras, Slovakia, with a focus on the isolation and characterization of psychrophilic microorganisms with potential biotechnological applications. Water and sediment samples were collected from five alpine lakes and cultured at two temperatures 20 °C and 4 °C to differentiate. Using MALDI-TOF mass spectrometry, 107 bacterial isolates were identified. At 20 °C, the predominant genera included Serratia, Bacillus and Pseudomonas, with a notable abundance of potentially pathogenic Enterobacteriaceae in Slavkovské pleso, suggesting anthropogenic influence. In contrast, cultivation at 4 °C yielded a distinct spectrum of psychrophilic taxa, including Pseudomonas antarctica, Exiguobacterium sibiricum and Janthinobacterium lividum — species known for their ecological resilience and functional potential in cold environments. The observed microbial profiles also provide insights into how environmental and anthropogenic factors shape microbiome composition in fragile alpine ecosystems. By highlighting the functional traits of psychrophiles, this work contributes to the broader understanding of microbiome adaptation, resilience and their prospective use in sustainable biotechnology.