Evaluating the impact of bee bread extract on transcriptional profiles in primary rat osteoblasts
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Súbory
Dátum
2025
Názov časopisu
ISSN časopisu
Názov zväzku
Vydavateľ
Slovak University of Agriculture : Nitra
ISBN
978-80-552-2829-7
978-80-552-2830-3
978-80-552-2830-3
ISSN
Abstrakt
Bee Bread (BB) is a fermented natural product rich in bioactive compounds with antioxidant and antimicrobial properties. Research in a rat model showed its potential to enhance diabetic bone structure, suggesting benefits for bone disorders. The aim of this in vitro study was to analyse the effects of BB extract on gene expression in osteoblasts. BB extract was prepared via methanol extraction, filtration, and evaporation, then dissolved in cell culture medium. It was applied to rat osteoblasts at 10, 100, and 200 μg/ml, with untreated medium as a control. After 72 hours, mRNA was extracted, cDNA synthesized, and the expression of 15 target genes (RUNX2, BMP2, BMP7, TGFB1, TGFBR1, BGLAP, ALPL, SPP1, VDR, COL1A1, IBSP, CDH11, VEGFA, TNFSF11, TNFRSF11B) analyzed using real-time PCR, with five reference genes (TBP, HPRT1, PGK1, B2M, LDHA). BB extract upregulated RUNX2, BMP7, TGFBR1, and ALPL in all groups. Dose-dependent effects included increased TGFB1, BMP2, and VDR in BB10; CDH11 in BB10 and BB100; TNFRSF11B in BB100 and BB200; and BGLAP and VEGFA in BB200. SPP1 was downregulated in BB10 and BB100, while TNFSF11, COL1A1, and IBSP remained unchanged. These findings indicate BB extract promotes osteoblast differentiation, bone mineralization, and osteogenesis while inhibiting bone resorption, demonstrating its potential for bone health and therapy.
Popis
In: Animal Biotechnology 2025 : winter school : programme and abstracts / rec. Ján Rafay, Sale Dragin. 1. vyd. Nitra : Slovak University of Agriculture, 2025. ISBN 978-80-552-2829-7. Pp. 72-72.
Kľúčové slová
liečivá, pharmaceuticals, včelí chlieb, bee bread, osteoblasty, osteoblasts
Výstup z projektu
KEGA 012UKF-4/2023 Implementácia inovácií do edukačného procesu predmetov všeobecná a špeciálna histológia živočíchov s orientáciou na prax
KEGA 023UKF-4/2025 Integrovaný prístup k výučbe molekulárno-biologických technológií s orientáciou pre potreby praxe: Praktická molekulárna biológia v študijnom programe biológia
Citácia
Práva a licenčné podmienky
CC BY-NC Creative Commons Attribution-NonCommercial 4.0. International
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess